Nucleoside Diphosphate Prodrugs

Synthesis of Nucleoside Phosphate and Phosphonate Prodrugs

ldentification as a Nucleoside Diphosphate Kinase'

A low molecular mas (18 kD) phosphoprotein (pp18) was characterized and purified from cultured sugarcane (Saccharum officinarum 1.) cell line H50-7209. Autophosphorylation assays were used to detect pp18 after separation by sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PACE). Only pp18 was detected by a brief in situ phosphorylation method, whereas additional putative protein ki...

Nucleoside diphosphate kinase from Escherichia coli.

Nucleoside diphosphate (NDP) kinase from Escherichia coli was purified to homogeneity and was crystallized. Gel filtration analysis of the purified enzyme indicated that it forms a tetramer. The enzyme was phosphorylated with [gamma-32P]ATP, and the pH stability profile of the phosphoenzyme indicated that two different amino acid residues were phosphorylated. Both a histidine residue and serine...

Hydroxyurea and Escherichia coli nucleoside diphosphate reductase.

M). The cultures were incubated at 37° for 40 mm (“Pretreatment―), whereupon the bacteria were washed and resuspended in fresh Medium HA. Portions from each culture were distributed into flasks containing premeasured amounts of the various drugs (“Additions―). After receiving cytidine-3H (2 @ig/ml; 0.13 i@Ci/ml),the cultures were incubated with aeration at 37°for 20 mm and quadr.uplic...

Microtubules and nucleoside diphosphate kinase. Nucleoside diphosphate kinase binds to co-purifying contaminants rather than to microtubule proteins.

Nucleoside diphosphate (NDP) kinase has been postulated to generate GTP from the GDP bound to tubulin. The purified chick brain enzyme was studied with respect to its kinetic parameters, and the protein-protein interactions between the NDP kinase and tubulin were examined. No specific interaction is observed between the enzyme and assembled microtubules, tubulin dimers, or tubulin-microtubule-a...